What is the catalog number for Bcl-2 Antibody?

The catalog number for Bcl-2 Antibody is V4581-20UG.

What sizes are available for Bcl-2 Antibody?

Bcl-2 Antibody is available in the following size(s): 20 µg.

Is Bcl-2 Antibody in stock?

Bcl-2 Antibody is currently made to order / not held in immediate stock. Contact us for lead time.

Bcl-2 Antibody

This antibody recognizes a protein of 25-26kDa, identified as the Bcl-2 alpha oncoprotein. It shows no cross-reaction with Bcl-x or Bax protein. Expression of Bcl-2 alpha oncoprotein inhibits the programmed cell death (apoptosis) . In most follicular lymphomas, neoplastic germinal centers express high levels of Bcl-2 alpha protein, whereas the normal or hyperplastic germinal centers are negative.Consequently, this antibody is valuable when distinguishing between reactive and neoplastic follicular proliferation in lymph node biopsies. It may also be used in distinguishing between those follicular lymphomas that express bcl-2 protein and the small number in which the neoplastic cells are bcl-2 negative.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

UniProt

P10415

Host

Mouse

Reactivity

Human

Immunogen

A recombinant partial protein sequence (within amino acids 1-200) from the human protein was used as the immunogen for the Bcl-2 antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

BCL2/6915

Applications

IHC-P

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the Bcl-2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This Bcl-2 antibody is available for research use only.

Storage Conditions

Aliquot the Bcl-2 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the Bcl-2 antibody should be determined by the researcher.

Location

Cytoplasm, nuclear membrane

Image Legend

IHC staining of FFPE human tonsil tissue with Bcl-2 antibody (clone BCL2/6915) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.