Desmoglein-3 Antibody

Recognizes a protein of 130kDa, identified as Desmoglein-3 (DSG3) . This mAb is highly specific to Desmoglein-3 and does not cross-react with other members of the Desmoglein-family. DSG3 is a calcium-binding transmembrane glycoprotein component of desmosomes in vertebrate epithelial cells. Desmosomes are cell-cell junctions between epithelial, myocardial, and certain other cell types. Currently, three desmoglein subfamily members are identified and all are members of the cadherin cell adhesion molecule superfamily.

Product Specifications

CAS Number

9000-83-3

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

UniProt

P32926

Host

Rabbit

Reactivity

Human

Immunogen

A recombinant partial protein sequence (within amino acids 300-500) from the human protein was used as the immunogen for the Desmoglein-3 antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG κ

Clone

DSG3/8252R

Type

Recombinant

Applications

IHC-P

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the Desmoglein-3 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This Desmoglein-3 antibody is available for research use only.

Storage Conditions

Aliquot the Desmoglein-3 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the Desmoglein-3 antibody should be determined by the researcher.

Location

Cell surface

Image Legend

IHC staining of FFPE human skin tissue with Desmoglein-3 antibody (clone DSG3/8252R) . Inset: PBS used in place of primary Ab (secondary Ab negative control) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.