Desmoglein 1 Antibody / DSG1

Recognizes a protein of ~150kDa, identified as Desmoglein-1 (DSG1) . Desmoglein-1 is a member of the desmosomal cadherin family. Desmosomes are intercellular adhering junctions that represent cell surface attachment sites for intermediate filament. Desmocollins and desmogleins are the main desmosomal transmembrane proteins. Desmogleins consist of Dsg1, Dsg2, Dsg3, and Dsg4 isoforms.Within the desmosome, the extracellular domain of desmoglein is essential for calcium dependent heterophilic binding to the desmocollins, whereas the intracellular domain is essential for binding to the desmosomal plaque protein, plakoglobin. Desmoglein 1 is synthesized exclusively in the suprabasal layers. Intact and functionally active desmoglein 1 is essential to epidermal integrity.

Product Specifications

CAS Number

9000-83-3

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

UniProt

Q02413

Host

Rabbit

Reactivity

Human

Immunogen

Recombinant human DSG1 protein was used as the immunogen for the Desmoglein 1 antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG κ

Clone

DSG1/8576R

Type

Recombinant

Applications

IHC-P

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the Desmoglein 1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This Desmoglein 1 antibody is available for research use only.

Storage Conditions

Aliquot the Desmoglein 1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the Desmoglein 1 antibody should be determined by the researcher.

Location

Cell surface

Image Legend

IHC staining of FFPE human skin tissue with Desmoglein 1 antibody (clone DSG1/8576R) . HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.