SMAD4 Antibody

SMAD4 is implicated as a tumor suppressor since it is inactivated in more than half of pancreatic carcinomas and to a lesser extent in a variety of other cancers. The lack of SMAD4 expression is present in approximately 80% of cases of pancreatic adenocarcinoma, but rarely in endometrial (0%), colorectal (0%), ovarian (3%), lung (0%), breast (2%) adenocarcinomas, and malignant melanoma (4%) . SMAD4is an important marker for confirming a diagnosis of pancreatic adenocarcinoma.

Product Specifications

CAS Number

9000-83-3

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

UniProt

Q13485

Host

Rabbit

Reactivity

Human

Immunogen

A recombinant partial protein sequence (within amino acids 400-552) from the human protein was used as the immunogen for the SMAD4 antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG κ

Clone

SMAD4/7905R

Type

Recombinant

Applications

IHC-P

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the SMAD4 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This SMAD4 antibody is available for research use only.

Storage Conditions

Aliquot the SMAD4 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the SMAD4 antibody should be determined by the researcher.

Location

Nucleus, Cytoplasm

Image Legend

IHC staining of FFPE human salivary gland tissue with SMAD4 / DPC4 antibody (clone SMAD4/7905R) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.