TIMP2 Antibody

It recognizes a protein of 21kDa, identified as tissue inhibitor of metalloproteinases-2 (TIMP-2) . It is closely related to TIMP-1 and shows the highest binding affinity to both the latent (pro) and active forms of 72kDa Type IV collagenase (also known as MMP-2 or gelatinase A) . It also has affinity for the active form of 92kDa Type IV collagenase (also known as MMP-9 or gelatinase B) . TIMP-2 inhibits the proteolytic invasiveness of tumor cells and normal placental trophoblast cells.

Product Specifications

CAS Number

9000-83-3

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

UniProt

P16035

Host

Rabbit

Reactivity

Human

Immunogen

A recombinant fragment of human TIMP2 protein was used as the immunogen for the TIMP2 antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG κ

Clone

TIMP2/8193R

Type

Recombinant

Applications

IHC-P

Purity

Protein A/G affinity

Format

Purified

Buffer

1 mg/ml in 1X PBS; BSA free, sodium azide free

Reconstitution

Aliquot the TIMP2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This TIMP2 antibody is available for research use only.

Storage Conditions

Aliquot the TIMP2 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

1 mg/mL in 1X PBS; BSA free, sodium azide free

Applications Notes

Optimal dilution of the TIMP2 antibody should be determined by the researcher.

Location

Cytoplasm, Cell surface, Secreted

Image Legend

IHC staining of FFPE human bladder carcinoma tissue with TIMP2 antibody (clone TIMP2/8193R) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.