Growth Hormone Antibody / GH1

Pituitary growth hormone (GH) plays a crucial role in stimulating and controlling the growth, metabolism and differentiation of many mammalian cell types by modulating the synthesis of multiple mRNA species. These effects are mediated by the binding of GH to its membrane-bound receptor, GHR, and involve a phosphorylation cascade that results in the modulation of numerous signaling pathways. GH is synthesized by acidophilic or somatotropic cells of the anterior pituitary gland. Anti-GH is a useful marker in classification of pituitary tumors and the study of pituitary disease (acromegaly) .

Product Specifications

CAS Number

9000-83-3

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

UniProt

P01241

Host

Rabbit

Reactivity

Human

Immunogen

Recombinant full-length human GH1 protein was used as the immunogen for the Growth Hormone antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG κ

Clone

GH/8215R

Type

Recombinant

Applications

IHC-P

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the Growth Hormone antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This Growth Hormone antibody is available for research use only.

Storage Conditions

Aliquot the Growth Hormone antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the Growth Hormone antibody should be determined by the researcher.

Location

Cytoplasm

Image Legend

IHC staining of FFPE human placental tissue with Growth Hormone antibody (clone GH/8215R) . Inset: PBS used in place of primary Ab (secondary Ab negative control) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.