N-Cadherin Antibody / CDH2 / Cadherin 2
Recognizes a protein of ~140kDa, identified as N-Cadherin (NCAD), also known as CD325. NCAD is a member of the Cadherin superfamily, and consists of five extracellular repeats, a transmembrane domain and a cytoplasmic domain. CD325 deficient mice die at day 10 of gestation and embryos display major heart defects and malformed neural tubes and somites. Consistent with this, CD325 has been implicated in several aspects of cardiac development including the precardiac mesoderm, establishment of left-right symmetry and cardiac looping morphogenesis. Furthermore, CD325 is normally involved in inducing cell cycle arrest and its expression is frequently deregulated in cancer cells. Studies have linked N-cadherin to cancer metastasis by showing the aggressive tumor cells had preferentially turned on N-cadherin as opposed to E- or P-cadherin.
Product Specifications
CAS Number
9000-83-3
Specifications
Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT, Western blot: 2-4 µg/mL
UniProt
P19022
Host
Rabbit
Reactivity
Human
Immunogen
A recombinant partial protein sequence (within amino acids 605-905) from the human protein was used as the immunogen for the N-Cadherin antibody.
Clonality
Recombinant Monoclonal
Isotype
IgG κ
Clone
CDH2/8998R
Type
Recombinant
Applications
IHC-P, WB
Purity
Protein A/G affinity
Format
Purified
Buffer
1 mg/ml in 1X PBS; BSA free, sodium azide free
Reconstitution
Aliquot the N-Cadherin antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This N-Cadherin antibody is available for research use only.
Storage Conditions
Aliquot the N-Cadherin antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
1 mg/mL in 1X PBS; BSA free, sodium azide free
Applications Notes
Optimal dilution of the N-Cadherin antibody should be determined by the researcher.
Location
Cell surface
Image Legend
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