N-Cadherin Antibody / CDH2 / Cadherin 2

Recognizes a protein of ~140kDa, identified as N-Cadherin (NCAD), also known as CD325. NCAD is a member of the Cadherin superfamily, and consists of five extracellular repeats, a transmembrane domain and a cytoplasmic domain. CD325 deficient mice die at day 10 of gestation and embryos display major heart defects and malformed neural tubes and somites. Consistent with this, CD325 has been implicated in several aspects of cardiac development including the precardiac mesoderm, establishment of left-right symmetry and cardiac looping morphogenesis. Furthermore, CD325 is normally involved in inducing cell cycle arrest and its expression is frequently deregulated in cancer cells. Studies have linked N-cadherin to cancer metastasis by showing the aggressive tumor cells had preferentially turned on N-cadherin as opposed to E- or P-cadherin.

Product Specifications

CAS Number

9000-83-3

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT, Western blot: 2-4 µg/mL

UniProt

P19022

Host

Rabbit

Reactivity

Human

Immunogen

A recombinant partial protein sequence (within amino acids 605-905) from the human protein was used as the immunogen for the N-Cadherin antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG κ

Clone

CDH2/8998R

Type

Recombinant

Applications

IHC-P, WB

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the N-Cadherin antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This N-Cadherin antibody is available for research use only.

Storage Conditions

Aliquot the N-Cadherin antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the N-Cadherin antibody should be determined by the researcher.

Location

Cell surface

Image Legend

Western blot testing of human heart tissue lysate with N-Cadherin antibody (clone CDH2/8998R) . Predicted molecular weight ~100 kDa (unmodified), 125-140 kDa (modified) .