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MPO Antibody / Myeloperoxidase

Myeloperoxidase (MPO) also called the peroxidase (POD), is an important marker of bone marrow cells. It is one of the members of the family of heme peroxidase super existing in myeloid cells (mainly neutrophils and monocytes of aniline blue particles) . With the deepening of the research on MPO, MPO gene polymorphism has been found to lead to individual for some disease susceptibility differences, with a variety of human development is closely related to the occurrence of diseases. The antibody reacts with neutrophil granulocytes and monocytes in blood and with precursors of granulocytes in the bone marrow. The antibody is useful as an aid for classification of neoplastic tissue, i.e. myeloblasts and immature myeloid cells of acute myelogenous leukemia, progranulocytic leukemia, monomyelocytic leukemia, erythroleukemia and myeloblastoma.

Product Specifications

CAS Number

9000-83-3

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

UniProt

P05164

Host

Rabbit

Reactivity

Human

Immunogen

A recombinant fragment of human MPO protein was used as the immunogen for the MPO antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG κ

Clone

MPO/8290R

Type

Recombinant

Applications

IHC-P

Purity

Protein A/G affinity

Format

Purified

Buffer

1 mg/ml in 1X PBS; BSA free, sodium azide free

Reconstitution

Aliquot the MPO antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This MPO antibody is available for research use only.

Storage Conditions

Aliquot the MPO antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

1 mg/mL in 1X PBS; BSA free, sodium azide free

Applications Notes

Optimal dilution of the MPO antibody should be determined by the researcher.

Location

Cytoplasm

Image Legend

IHC staining of FFPE human spleen tissue with MPO antibody (clone MPO/8290R) . Inset: PBS used in place of primary Ab (secondary Ab negative control) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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