CD271 Antibody / NGF Receptor / p75NTR

It recognizes a glycoprotein of 75kDa, identified as low affinity Nerve Growth Factor (NGF) Receptor (p75NGFR) or Neurotrophin Receptor (p75NTR) . NGFR is expressed in various neural crest cells and their tumors such as melanocytes, melanomas, neuroblastomas, pheochromocytomas and neurofibromas. Reportedly, anti-NGFR is a reliable marker for desmoplastic and neurotropic melanomas. NGFR is expressed in mature non-neural cells such as perivascular cells, dental pulp cells, lymphoidal follicular dendritic cells, basal epithelium of oral mucosa and hair follicles, prostate basal cells, and myoepithelial cells. Anti-NGFR stains the myoepithelial cells of breast ducts and intra-lobular fibroblasts of breast ducts.

Product Specifications

CAS Number

9000-83-3

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

UniProt

P08138

Host

Rabbit

Reactivity

Human

Immunogen

Recombinant human full-length protein was used as the immunogen for the CD271 antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG κ

Clone

NGFR/8590R

Type

Recombinant

Applications

IHC-P

Purity

Protein A/G affinity

Format

Purified

Buffer

1 mg/ml in 1X PBS; BSA free, sodium azide free

Reconstitution

Aliquot the CD271 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This CD271 antibody is available for research use only.

Storage Conditions

Aliquot the CD271 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

1 mg/mL in 1X PBS; BSA free, sodium azide free

Applications Notes

Optimal dilution of the CD271 antibody should be determined by the researcher.

Location

Cell surface, Cytoplasm

Image Legend

IHC staining of FFPE human spleen tissue with CD271 antibody (clone NGFR/8590R) . Inset: PBS used in place of primary Ab (secondary Ab negative control) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.