NME2 Antibody / Nucleoside diphosphate kinase B / NDKB
The nm23 gene, a potential suppressor of metastasis, was originally identified by differential hybridization between two murine melanoma sub-lines, one with a high and the second with a low metastatic capacity. Highly metastatic sub-lines exhibit much lower levels of nm23 than less metastatic cells. Based on sequence analysis, nm23 appears highly related to nucleotide diphosphate kinases (NDP) . In humans, NDP kinases A and B are identical to two isotypes of human nm23 homologs, namely nm23-H1 and H2, respectively. nm23-H2 is identical in sequence to PuF, a transcription factor that binds to nuclease hypersensitive elements at positions 142 to 115 of the human c-Myc promotor.
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT
UniProt
P22392
Host
Mouse
Reactivity
Human
Immunogen
Recombinant full-length human protein was used as the immunogen for the NME2 antibody.
Clonality
Monoclonal
Isotype
IgG2 κ
Clone
NME2/4160
Applications
WB, IHC-P
Purity
Protein A/G affinity
Format
Purified
Buffer
1 mg/ml in 1X PBS; BSA free, sodium azide free
Reconstitution
Aliquot the NME2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This NME2 antibody is available for research use only.
Storage Conditions
Aliquot the NME2 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
1 mg/mL in 1X PBS; BSA free, sodium azide free
Applications Notes
Optimal dilution of the NME2 antibody should be determined by the researcher.
Location
Cytoplasm, Nucleus
Image Legend
Western blot testing of human 293T and MCF-7 cell lysate with NME2 antibody (clone NME2/4160) . Predicted molecular weight ~17 kDa.
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