NME2 Antibody / Nucleoside diphosphate kinase B / NDKB
The nm23 gene, a potential suppressor of metastasis, was originally identified by differential hybridization between two murine melanoma sub-lines, one with a high and the second with a low metastatic capacity. Highly metastatic sub-lines exhibit much lower levels of nm23 than less metastatic cells. Based on sequence analysis, nm23 appears highly related to nucleotide diphosphate kinases (NDP) . In humans, NDP kinases A and B are identical to two isotypes of human nm23 homologs, namely nm23-H1 and H2, respectively. nm23-H2 is identical in sequence to PuF, a transcription factor that binds to nuclease hypersensitive elements at positions 142 to 115 of the human c-Myc promotor.
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT
UniProt
P22392
Host
Mouse
Reactivity
Human
Immunogen
Recombinant full-length human protein was used as the immunogen for the NME2 antibody.
Clonality
Monoclonal
Isotype
IgG2 κ
Clone
NME2/4160
Applications
WB, IHC-P
Purity
Protein A/G affinity
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Aliquot the NME2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This NME2 antibody is available for research use only.
Storage Conditions
Aliquot the NME2 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide
Applications Notes
Optimal dilution of the NME2 antibody should be determined by the researcher.
Location
Cytoplasm, Nucleus
Image Legend
Western blot testing of human 293T and MCF-7 cell lysate with NME2 antibody (clone NME2/4160) . Predicted molecular weight ~17 kDa.
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