Pan Cytokeratin Antibody Cocktail (Acidic + Basic)

This antibody cocktail recognizes acidic (Type I or LMW) and basic (Type II or HMW) cytokeratins, with 67kDa (CK1) ; 64kDa (CK3) ; 59kDa (CK4) ; 58kDa (CK5) ; 56kDa (CK6) ; 55kDa (CK7) ; 52kDa (CK8) ; 56.5kDa (CK10) ; 53kDa (CK13) ; 50kDa (CK14) ; 50kDa (CK15) ; 48kDa (CK16) ; 46kDa (CK17) ; 45kDa (CK18), 40kDa (CK19) and 46kDa (CK20) . Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. This antibody is a broad-spectrum anti pan-cytokeratin antibody cocktail, which differentiates epithelial tumors from non-epithelial tumors e.g. squamous vs. adenocarcinoma of the lung, liver carcinoma, breast cancer, and esophageal cancer. It is useful in characterizing the source of various neoplasms and to study the distribution of cytokeratin containing cells in epithelia during normal development and during the development of epithelial neoplasms.

Product Specifications

CAS Number

9000-83-3

Specifications

Flow cytometry: 0.5-1ug/million cells, Immunofluorescence: 1-2 µg/mL, Western blot: 0.5-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 minutes at RT

UniProt

Q7Z794, Q01546

Host

Rabbit

Reactivity

Human

Immunogen

Recombinant fragments and/or synthetic peptides of human Cytokeratin proteins were used as the immunogen for the Pan Cytokeratin antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG κ

Clone

MonoPoly/4999R

Type

Recombinant

Applications

FACS, IF, WB, IHC-P

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the Pan Cytokeratin antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This Pan Cytokeratin antibody is available for research use only.

Storage Conditions

Aliquot the Pan Cytokeratin antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the Pan Cytokeratin antibody should be determined by the researcher.

Location

Cytoplasm

Image Legend

IHC staining of FFPE human tonsil tissue with Pan Cytokeratin antibody (clone MonoPoly/4999R) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.