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Type I Cytokeratin Antibody / Acidic / LMW

The keratins are the typical intermediate filament proteins of epithelia, showing an outstanding degree of molecular diversity. Heteropolymeric filaments are formed by pairing of type I and type II molecules. In humans 54 functional keratin genes exist. They are expressed in highly specific patterns related to the epithelial type and stage of cellular differentiation.

Product Specifications

CAS Number

9000-83-3

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 minutes at RT

UniProt

Q7Z794

Host

Rabbit

Reactivity

Human

Immunogen

A synthetic peptide corresponding to human Type I Cytokeratin proteins was used as the immunogen for the Type I Cytokeratin antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG

Clone

KRTL/7784R

Type

Recombinant

Applications

IHC-P

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the Type I Cytokeratin antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This Type I Cytokeratin antibody is available for research use only.

Storage Conditions

Aliquot the Type I Cytokeratin antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the Type I Cytokeratin antibody should be determined by the researcher.

Location

Cytoplasm

Image Legend

IHC staining of FFPE human prostate carcinoma tissue with Type I Cytokeratin antibody (clone KRTL/7784R) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

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