Topoisomerase II alpha Antibody / TOP2A
The topoisomerase II-Alpha isoform is a 170kDa nuclear protein and plays an important role in DNA synthesis and RNA transcription, as well as chromosomal segregation during mitosis. Topoisomerase II-Alpha is reported to be a sensitive and specific marker of late S-, G2- & M-phases in transformed and developmentally regulated normal cells, and has been shown to be over-expressed in many human cancers. Decreased expression of Topoisomerase II-Alpha is the predominant mechanism of resistance to several chemotherapeutic agents.
Product Specifications
CAS Number
9000-83-3
Specifications
Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 minutes at RT
UniProt
P11388
Host
Rabbit
Reactivity
Human, Dog
Immunogen
Clonality
Recombinant Monoclonal
Isotype
IgG κ
Clone
TOP2A/7169R
Type
Recombinant
Applications
IHC-P
Purity
Protein A/G affinity
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Aliquot the Topoisomerase II alpha antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This Topoisomerase II alpha antibody is available for research use only.
Storage Conditions
Aliquot the Topoisomerase II alpha antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide
Applications Notes
Optimal dilution of the Topoisomerase II alpha antibody should be determined by the researcher.
Location
Nucleus
Image Legend
Documents
Frequently Asked Questions
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