Cytokeratin 20 Antibody
This mAb recognizes an intermediate filament protein of 46kDa, identified as cytokeratin 20 (KRT20) . KRT20 is abundantly expressed in goblet cells and enterocytes of the gastrointestinal tract. It is a useful marker of pancreatic and colorectal cancer. KRT20 is expressed under normal, hyperplastic and neoplastic conditions. It has been detected in adenocarcinomas of the colon, stomach and biliary tract. Breast carcinomas are generally non-reactive.
Product Specifications
CAS Number
9000-83-3
Specifications
Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 minutes at RT, Western blot: 2-4 µg/mL
UniProt
P35900
Host
Rabbit
Reactivity
Human
Immunogen
A recombinant fragment of human KRT20 protein (within amino acids 324-424) was used as the immunogen for the Cytokeratin 20 antibody.
Clonality
Recombinant Monoclonal
Isotype
IgG κ
Clone
KRT20/7805R
Type
Recombinant
Applications
IHC-P, WB
Purity
Protein A/G affinity
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Aliquot the Cytokeratin 20 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This Cytokeratin 20 antibody is available for research use only.
Storage Conditions
Aliquot the Cytokeratin 20 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide
Applications Notes
Optimal dilution of the Cytokeratin 20 antibody should be determined by the researcher.
Location
Cytoplasm
Image Legend
Western blot testing of human HT29 cell lysate with Cytokeratin 20 antibody. Predicted molecular weight ~46 kDa.
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