Cytokeratin 10 Antibody
This mAb recognizes a protein of 56.5kDa, identified as cytokeratin 10 (CK10) . CK10 is expressed in all suprabasal layers of the epidermis. In the epidermis, expression of CK10 strictly parallels the extent of differentiation; it is absent in the basal layer, appears in the first suprabasal layers and increases in concentration towards the granular layer. However, CK10 is rarely detected in early stages of vulvar squamous carcinomas (tumors less than 2 cm, clinical stage I) regardless of the tumor grade. In larger and more advanced tumors (greater than 2 cm, clinical stages II and III), CK10 is detected very frequently. Expression of CK10 is related to maturation of malignant keratinocytes, being preferentially detected in more-differentiated parts.
Product Specifications
CAS Number
9000-83-3
Specifications
Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 minutes at RT
UniProt
P13645
Host
Rabbit
Reactivity
Human
Immunogen
A recombinant human fragment (within amino acids 500-600) of human protein was used as the immunogen for the Cytokeratin 10 antibody.
Clonality
Recombinant Monoclonal
Isotype
IgG κ
Clone
KRT10/7804R
Type
Recombinant
Applications
IHC-P
Purity
Protein A/G affinity
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Aliquot the Cytokeratin 10 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This Cytokeratin 10 antibody is available for research use only.
Storage Conditions
Aliquot the Cytokeratin 10 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide
Applications Notes
Optimal dilution of the Cytokeratin 10 antibody should be determined by the researcher.
Location
Cytoplasmic
Image Legend
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