Glial Fibrillary Acidic Protein Antibody / GFAP

This mAb recognizes a protein of ~50kDa which is identified as Glial Fibrillary Acidic Protein (GFAP) . It shows no cross-reaction with other intermediate filament proteins. GFAP is specifically found in astroglia. GFAP is a very popular marker for localizing benign astrocyte and neoplastic cells of glial origin in the central nervous system.Antibody to GFAP is useful in differentiating primary gliomas from metastatic lesions in the brain and for documenting astrocytic differentiation in tumors outside the CNS.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT, Western blot: 2-4 µg/mL

UniProt

P14136

Host

Mouse

Reactivity

Human

Immunogen

A recombinant partial protein sequence (within amino acids 150-250) from the human protein was used as the immunogen for the Glial Fibrillary Acidic Protein antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

GFAP/6876

Applications

IHC-P, WB

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the Glial Fibrillary Acidic Protein antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This Glial Fibrillary Acidic Protein antibody is available for research use only.

Storage Conditions

Aliquot the Glial Fibrillary Acidic Protein antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the Glial Fibrillary Acidic Protein antibody should be determined by the researcher.

Location

Cytoplasm

Image Legend

IHC staining of FFPE human cerebellum tissue with Glial Fibrillary Acidic Protein antibody (clone GFAP/6876) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.