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Acidic Cytokeratin Antibody / LMW / Type I

This mAb recognizes the 56.5kDa (CK10) ; 50kDa (CK14) ; 50kDa (CK15) ; 48kDa (CK16) ; 40kDa (CK19) keratins of the acidic (Type I or LMW) subfamily. Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. The acidic keratins have molecular weights (MW) of 56.5, 55, 51, 50, 5048, 46, 45, and 40kDa.

Product Specifications

CAS Number

9000-83-3

Specifications

Immunohistochemistry (FFPE) : 0.5-1 µg/mL for 30 min at RT

UniProt

Q7Z794

Host

Mouse

Reactivity

Human

Immunogen

Human epidermal keratin was used as the immunogen for the Acidic Cytokeratin antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG1 k

Clone

RKRTL/8751

Type

Recombinant

Applications

IHC-P

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the Acidic Cytokeratin antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This Acidic Cytokeratin antibody is available for research use only.

Storage Conditions

Aliquot the Acidic Cytokeratin antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the Acidic Cytokeratin antibody should be determined by the researcher.

Location

Cytoplasm

Image Legend

IHC staining of FFPE human placental tissue with Acidic Cytokeratin antibody (clone rKRTL/8751) . Inset: PBS used in place of primary Ab (secondary Ab negative control) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

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