Recombinant TOP2A Antibody / Topoisomerase II alpha
It recognizes a 170kDa protein, which is identified as topoisomerase IIAlpha. It shows no cross-reaction with Topoisomerase II beta or I. Topo II Alpha plays important roles in synthesis and transcription of DNA as well as chromosomal segregation during mitosis. It is reported to be a sensitive and specific marker of late S-, G2- & M-phases in transformed and developmentally regulated normal cells. Topo II Alpha is also implicated in drug resistance of tumor cells and has been shown to be over-expressed in many human cancers. Decreased expression of Topo IIa is the predominant mechanism of resistance to several chemotherapeutic agents.
Product Specifications
CAS Number
9000-83-3
Specifications
Immunohistochemistry (FFPE) : 0.5-1 µg/mL for 30 minutes at RT
UniProt
P11388
Host
Mouse
Reactivity
Human
Immunogen
A recombinant human Topoisomerase II alpha protein fragment (aa1300-1500) was used as the immunogen for the recombinant TOP2A antibody.
Clonality
Recombinant Monoclonal
Isotype
IgG1 k
Clone
RTOP2A/8339
Type
Recombinant
Applications
IHC-P
Purity
Protein A/G affinity
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Aliquot the recombinant TOP2A antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This recombinant TOP2A antibody is available for research use only.
Storage Conditions
Aliquot the recombinant TOP2A antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide
Applications Notes
Optimal dilution of the recombinant TOP2A antibody should be determined by the researcher.
Location
Nucleus
Image Legend
Frequently Asked Questions
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