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Recombinant HER2 Antibody / ErbB2

C-erbB-2/HER-2 is a member of the EGFR family. This mAb is specific and shows minimal cross-reaction with other members of the EGFR-family. Receptors of this family are located on the plasma membrane and consist of an extracellular ligand-binding domain that is connected to a large intracellular domain by a single transmembrane sequence. c-erbB-2/HER-2 protein is over-expressed in a variety of carcinomas especially those of breast and ovary.

Product Specifications

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 minutes at RT

UniProt

P04626

Host

Rabbit

Reactivity

Human

Immunogen

A recombinant partial protein sequence (within amino acids 1055-1255) from the human protein was used as the immunogen for the recombinant HER2 antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG κ

Clone

ERBB2/7158R

Type

Recombinant

Applications

IHC-P

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the recombinant HER2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This recombinant HER2 antibody is available for research use only.

Storage Conditions

Aliquot the recombinant HER2 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Product Datasheet

https://www.nsjbio.com/tds-pdf/recombinant-her2-antibody-erbb2-erbb27158r-v4470

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the recombinant HER2 antibody should be determined by the researcher.

CAS Number

9000-83-3

Location

Cell surface

Image Legend

IHC staining of FFPE human breast carcinoma tissue with recombinant HER2 antibody (clone ERBB2/7158R) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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