Recombinant HER2 Antibody / ErbB2

C-erbB-2/HER-2 is a member of the EGFR family. This mAb is specific and shows minimal cross-reaction with other members of the EGFR-family. Receptors of this family are located on the plasma membrane and consist of an extracellular ligand-binding domain that is connected to a large intracellular domain by a single transmembrane sequence. c-erbB-2/HER-2 protein is over-expressed in a variety of carcinomas especially those of breast and ovary.

Product Specifications

CAS Number

9000-83-3

Specifications

Flow cytometry: 1-2ug/million cells, Immunofluorescence: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 minutes at RT

UniProt

P04626

Host

Rabbit

Reactivity

Human

Immunogen

A recombinant partial protein sequence (within amino acids 1055-1255) from the human protein was used as the immunogen for the recombinant HER2 antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG κ

Clone

ERBB2/8142R

Type

Recombinant

Applications

FACS, IF, IHC-P

Purity

Protein A/G affinity

Format

Purified

Buffer

1 mg/ml in 1X PBS; BSA free, sodium azide free

Reconstitution

Aliquot the recombinant HER2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This recombinant HER2 antibody is available for research use only.

Storage Conditions

Aliquot the recombinant HER2 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

1 mg/mL in 1X PBS; BSA free, sodium azide free

Applications Notes

Optimal dilution of the recombinant HER2 antibody should be determined by the researcher.

Location

Cell surface

Image Legend

IHC staining of FFPE human breast carcinoma tissue with recombinant HER2 antibody (clone ERBB2/8142R) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.