ErbB2 Antibody / HER2 / Extracellular region

Recognizes a protein of 185kDa, which is identified as c-erbB-2/HER-2/neu. Its epitope is localized in the extracellular domain. C-erbB-2/HER-2 is a member of the EGFR family. This mAb is specific and shows minimal cross-reaction with other members of the EGFR-family. Receptors of this family are located on the plasma membrane and consist of an extracellular ligand-binding domain that is connected to a large intracellular domain by a single transmembrane sequence. c-erbB-2/HER-2 protein is over-expressed in a variety of carcinomas especially those of breast and ovary.

Product Specifications

CAS Number

9000-83-3

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 minutes at RT

UniProt

P04626

Host

Mouse

Reactivity

Human

Immunogen

Recombinant protein encoding the extracellular domain of the human protein was used as the immunogen for the ErbB2 antibody.

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

ERBB2/6637

Type

Recombinant

Applications

IHC-P

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the recombinant ErbB2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This ErbB2 antibody is available for research use only.

Storage Conditions

Aliquot the ErbB2 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the ErbB2 antibody should be determined by the researcher.

Location

Cell Surface

Image Legend

IHC staining of FFPE human breast carcinoma tissue stained with ErbB2 antibody (clone ERBB2/6637) at 2ug/ml. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.