Recombinant 14-3-3E Antibody / YWHAE
Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathway. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. It interacts with CDC25 phosphatases, RAF1 and IRS1 proteins, suggesting its role in diverse biochemical activities related to signal transduction, such as cell division and regulation of insulin sensitivity. It has also been implicated in the pathogenesis of small cell lung cancer. The YWHAE gene which encodes 14-3-3E has also been identified as a possible susceptibility gene for schizophrenia.
Product Specifications
CAS Number
9000-83-3
Specifications
Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT, Western blot: 2-4 µg/mL
UniProt
P62258
Host
Rabbit
Reactivity
Human
Immunogen
Recombinant human full-length protein was used as the immunogen for the recombinant 14-3-3E antibody.
Clonality
Recombinant Monoclonal
Isotype
IgG κ
Clone
YWHAE/8636R
Type
Recombinant
Applications
IHC-P, WB
Purity
Protein A/G affinity
Format
Purified
Buffer
1 mg/ml in 1X PBS; BSA free, sodium azide free
Reconstitution
Aliquot the recombinant 14-3-3E antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This recombinant 14-3-3E antibody is available for research use only.
Storage Conditions
Aliquot the recombinant 14-3-3E antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
1 mg/mL in 1X PBS; BSA free, sodium azide free
Applications Notes
Optimal dilution of the recombinant 14-3-3E antibody should be determined by the researcher.
Location
Cytoplasm, Melanosome
Image Legend
Western blot testing of human 293T, PC-3 and A431 cell lysate with recombinant 14-3-3E antibody. Predicted molecular weight ~28 kDa.
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