14-3-3 epsilon Antibody / YWHAE
Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathway. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. It interacts with CDC25 phosphatases, RAF1 and IRS1 proteins, suggesting its role in diverse biochemical activities related to signal transduction, such as cell division and regulation of insulin sensitivity. It has also been implicated in the pathogenesis of small cell lung cancer. The YWHAE gene which encodes 14-3-3E has also been identified as a possible susceptibility gene for schizophrenia.
Product Specifications
CAS Number
9000-83-3
Specifications
Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 minutes at RT
UniProt
P62268
Host
Rabbit
Reactivity
Human
Immunogen
Recombinant human full-length protein was used as the immunogen for the 14-3-3 epsilon antibody.
Clonality
Recombinant Monoclonal
Isotype
IgG κ
Clone
YWHAE/8309R
Type
Recombinant
Applications
IHC-P
Purity
Protein A/G affinity
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Aliquot the 14-3-3 epsilon antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This 14-3-3 epsilon antibody is available for research use only.
Storage Conditions
Aliquot the 14-3-3 epsilon antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide
Applications Notes
Optimal dilution of the 14-3-3 epsilon antibody should be determined by the researcher.
Location
Cytoplasm, Melanosome
Image Legend
SDS-PAGE analysis of purified, BSA-free 14-3-3 epsilon antibody (clone YWHAE/8309R) as confirmation of integrity and purity.
Frequently Asked Questions
Explore Other Products
Browse additional items from our catalog
CD31 / PECAM-1 (158-2B3), CF740 conjugate, 0.1mg/mL
Cytokeratin 19 (KRT19) (Pancreatic Stem Cell Marker) (rKRT19/799), CF405S conjugate, 0.1mg/mL
ATRX / RAD54 (Alpha Thalassemia Mental Retardation) (ATRX/2900R), CF640R conjugate, 0.1mg/mL
