Recombinant TUBB3 Antibody / Beta Tubulin

This gene encodes a class III member of the beta tubulin protein family. Beta tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize and assemble to form microtubules. This protein is primarily expressed in neurons and may be involved in neurogenesis and axon guidance and maintenance. Mutations in this gene are the cause of congenital fibrosis of the extraocular muscles type 3. In adults, tubulin beta 3 (TUBB3) is primarily expressed in neurons and is commonly used as a neuronal marker. It plays an important role in neuronal cell proliferation and differentiation.

Product Specifications

CAS Number

9000-83-3

Specifications

Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 minutes at RT

UniProt

Q13509

Host

Mouse

Reactivity

Human

Immunogen

A recombinant partial protein sequence (within amino acids 250-450) from the human protein was used as the immunogen for the recombinant TUBB3 antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG1 k

Clone

RTUBB3/7405

Type

Recombinant

Applications

WB, IHC-P

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the recombinant TUBB3 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This recombinant TUBB3 antibody is available for research use only.

Storage Conditions

Aliquot the recombinant TUBB3 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the recombinant TUBB3 antibody should be determined by the researcher.

Location

Cytoplasm

Image Legend

IHC staining of FFPE human brain tissue with recombinant TUBB3 antibody (clone rTUBB3/7405) . Inset: PBS used in place of primary Ab (secondary Ab negative control) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.