Ksp-Cadherin Antibody / CDH16

This mAb recognizes a protein of 130kDa, identified as Ksp-cadherin. Cadherins form a superfamily of related glycoproteins that mediate calcium-dependent cell adhesion and transmit signals from the extracellular matrix to the cytoplasm. Cadherins have been implicated in embryogenesis, tissue morphogenesis, tissue structure maintenance, cell polarization, neoplastic invasiveness and metastasis, and membrane transport. It is suggested that Ksp-cadherin is a marker for terminal differentiation of the basolateral membranes of renal tubular epithelial cells. Within the kidney, Ksp-Cadherin is found exclusively in the basolateral membrane of renal tubular epithelial cells and collecting duct cells, and not in glomeruli, renal interstitial cells, or blood vessels. Ksp-Cadherin has been suggested to distinguish Chromophobe Renal-Cell Carcinoma from Oncocytoma.

Product Specifications

CAS Number

9000-83-3

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 minutes at RT

UniProt

O75309

Host

Rabbit

Reactivity

Human

Immunogen

A recombinant partial protein sequence (within amino acids 350-550) from the human protein was used as the immunogen for the Ksp-Cadherin antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG κ

Clone

CDH16/8800R

Type

Recombinant

Applications

IHC-P

Purity

Protein A affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the Ksp-Cadherin antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This Ksp-Cadherin antibody is available for research use only.

Storage Conditions

Aliquot the Ksp-Cadherin antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the Ksp-Cadherin antibody should be determined by the researcher.

Location

Cell surface, Cytoplasm

Image Legend

IHC staining of FFPE human kidney tissue with Ksp-Cadherin antibody (clone CDH16/8800R) at 2ug/ml. Inset: PBS used in place of primary Ab (secondary Ab negative control) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.