Matrix metalloproteinase 2 Antibody / MMP2

It recognizes a protein of 72kDa, which is identified as MMP2. The matrix metalloproteinases (MMP) are a family of peptidase enzymes responsible for the degradation of extracellular matrix components, including collagen, gelatin, Fibronectin, Laminin and proteoglycan. Transcription of MMP genes is differentially activated by phorbol ester, lipopolysaccharide (LPS) or staphylococcal enterotoxin B (SEB) . MMP catalysis requires both calcium and zinc. MMP-2 (also designated type IV collagenase) cleaves collagen types IV, V, VII and X and gelatin type I. Activation of MMP-2 secretion requires the Ras signaling pathway.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 minutes at RT

UniProt

P08253

Host

Mouse

Reactivity

Human

Immunogen

Recombinant human protein was used as the immunogen for the Matrix metalloproteinase 2 antibody.

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

MMP2/4586

Applications

IHC-P

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the Matrix metalloproteinase 2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This Matrix metalloproteinase 2 antibody is available for research use only.

Storage Conditions

Aliquot the Matrix metalloproteinase 2 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the Matrix metalloproteinase 2 antibody should be determined by the researcher.

Location

Cytoplasm, Cell Surface

Image Legend

IHC staining of FFPE human placental tissue with Matrix metalloproteinase 2 antibody (clone MMP2/4586) . Inset: PBS used in place of primary Ab (secondary Ab negative control) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.