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Recombinant MSLN Antibody / Mesothelin

Mesothelin is a 40kDa glycosyl-phosphatidylinositol-anchored glycoprotein cleaved from a 69kDa precursor protein. Mesothelin immunoreactivity is high in cancers of the ovary (serous papillary, endometrioid and undifferentiated) and pancreas, with less frequent staining seen in adenocarcinomas of the endometrium, lung and stomach/esophagus. Mesothelin is one of the most sensitive markers for mesothelioma.

Product Specifications

CAS Number

9000-83-3

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 minutes at RT, Western blot: 2-4 µg/mL

UniProt

Q13421

Host

Mouse

Reactivity

Human

Immunogen

A recombinant partial protein sequence (within amino acids 273-407) from the human protein was used as the immunogen for the recombinant MSLN antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG2b κ

Clone

RMSLN/8764

Type

Recombinant

Applications

IHC-P, WB

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the recombinant MSLN antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This recombinant MSLN antibody is available for research use only.

Storage Conditions

Aliquot the recombinant MSLN antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the recombinant MSLN antibody should be determined by the researcher.

Location

Cell Surface, Secreted

Image Legend

Western blot testing of human OVCAR-3 cell lysate with MSLN antibody. Predicted molecular weight ~70 kDa (precursor), ~40 kDa (processed form) .

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