Recombinant Mesothelin Antibody

Mesothelin is a 40kDa glycosyl-phosphatidylinositol-anchored glycoprotein cleaved from a 69kDa precursor protein. Mesothelin immunoreactivity is high in cancers of the ovary (serous papillary, endometrioid and undifferentiated) and pancreas, with less frequent staining seen in adenocarcinomas of the endometrium, lung and stomach/esophagus. Mesothelin is one of the most sensitive markers for mesothelioma.

Product Specifications

CAS Number

9000-83-3

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT, Western blot: 2-4 µg/mL

UniProt

Q13421

Host

Rabbit

Reactivity

Human

Immunogen

A recombinant partial protein sequence (within amino acids 273-407) from the human protein was used as the immunogen for the recombinant Mesothelin antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG κ

Clone

MSLN/8391R

Type

Recombinant

Applications

IHC-P, WB

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the recombinant Mesothelin antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This recombinant Mesothelin antibody is available for research use only.

Storage Conditions

Aliquot the recombinant Mesothelin antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the recombinant Mesothelin antibody should be determined by the researcher.

Location

Cell surface, Secreted

Image Legend

IHC staining of FFPE human ovarian cancer tissue with recombinant Mesothelin antibody (MSLN/8391R) . Inset: PBS used in place of primary Ab (secondary Ab negative control) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.