Recombinant BCMA Antibody

The B cell maturation protein (BCMA) is a type I integral membrane protein that belongs to the tumor necrosis factor receptor (TNF-R) superfamily. It is expressed as a 184 amino acid peptide that is expressed only in mature B-lymphocytes and is located on the cis part of the Golgi apparatus. BCMA shares significant homology with TACI (transmembrane activator) within the cysteine-rich domain. TACI has been shown to bind CAML, which induces activation of NFAT (nuclear factor of activated T cells) . Both BCMA and TACI have been shown to bind APRIL and TALL-1, which stimulate B cell proliferation in conjunction with other B-cell activators. When overexpressed, TALL-1 stimulates the development of systemic lupus erythaematosus (SLE) .

Product Specifications

CAS Number

9000-83-3

Specifications

ELISA (Order BSA-free format for coating), Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 minutes at RT

UniProt

Q02223

Host

Rabbit

Reactivity

Human

Immunogen

A recombinant human protein fragment (within amino acids 1-184) was used as the immunogen for the recombinant BCMA antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG κ

Clone

CD269/8276R

Type

Recombinant

Applications

ELISA, IHC-P

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the recombinant BCMA antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This recombinant BCMA antibody is available for research use only.

Storage Conditions

Aliquot the recombinant BCMA antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the recombinant BCMA antibody should be determined by the researcher.

Location

Cell surface

Image Legend

IHC staining of FFPE human tonsil tissue with recombinant BCMA antibody (clone CD269/8276R) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.