Recombinant GP2 Antibody / Glycoprotein 2 / ZAP75

GP2 (glycoprotein 2), also known as ZAP75, is a 537 amino acid secreted protein. It is an integral membrane protein that is secreted from intracellular zymogen granules and associates with the plasma membrane via glycosylphosphatidylinositol (GPI) linkage. GP2 is cleaved and then released into the pancreatic duct along with exocrine secretions. GP2 binds pathogens such as enterobacteria, thereby playing an important role in the innate immune response. The C-terminus of this protein is related to the C-terminus of the protein encoded by the neighboring gene, uromodulin (UMOD) . GP2 is also expressed on the apical plasma membrane of specialized microfold (M) cells among enterocytes and serves as a transcytotic receptor for mucosal antigens. M cells are considered a promising target for oral vaccination against various infectious diseases.

Product Specifications

CAS Number

9000-83-3

Specifications

ELISA: 2-4 mg/mL for coating (order BSA-free format), Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 minutes at RT

UniProt

P55259

Host

Mouse

Reactivity

Human

Immunogen

A recombinant fragment of human protein (within amino acids 35-179) was used as the immunogen for the recombinant GP2 antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG1 k

Clone

RGP2/8617

Type

Recombinant

Applications

ELISA, WB, IHC-P

Purity

Protein A/G affinity

Format

Purified

Buffer

1 mg/ml in 1X PBS; BSA free, sodium azide free

Reconstitution

Aliquot the recombinant GP2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This recombinant GP2 antibody is available for research use only.

Storage Conditions

Aliquot the recombinant GP2 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

1 mg/mL in 1X PBS; BSA free, sodium azide free

Applications Notes

Optimal dilution of the recombinant GP2 antibody should be determined by the researcher.

Location

Cell surface, Secreted, Cytoplasmic

Image Legend

IHC staining of FFPE human pancreas tissue with recombinant GP2 antibody (clone rGP2/8617) . Inset: PBS used in place of primary Ab (secondary Ab negative control) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.