Glycoprotein 2 Antibody / GP2 / ZAP75

GP2 (glycoprotein 2), also known as ZAP75, is a 537 amino acid secreted protein. It is an integral membrane protein that is secreted from intracellular zymogen granules and associates with the plasma membrane via glycosylphosphatidylinositol (GPI) linkage. GP2 is cleaved and then released into the pancreatic duct along with exocrine secretions. GP2 binds pathogens such as enterobacteria, thereby playing an important role in the innate immune response. The C-terminus of this protein is related to the C-terminus of the protein encoded by the neighboring gene, uromodulin (UMOD) . GP2 is also expressed on the apical plasma membrane of specialized microfold (M) cells among enterocytes and serves as a transcytotic receptor for mucosal antigens. M cells are considered a promising target for oral vaccination against various infectious diseases.

Product Specifications

CAS Number

9007-83-4

Specifications

ELISA: 2-4 mg/mL for coating (order BSA-free format), Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 minutes at RT

UniProt

P55259

Host

Mouse

Reactivity

Human

Immunogen

A recombinant fragment of human protein (within amino acids 35-179) was used as the immunogen for the Glycoprotein 2 antibody.

Clonality

Monoclonal

Isotype

IgG2c κ

Clone

GP2/3510

Applications

ELISA, WB, IHC-P

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the Glycoprotein 2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This Glycoprotein 2 antibody is available for research use only.

Storage Conditions

Aliquot the Glycoprotein 2 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the Glycoprotein 2 antibody should be determined by the researcher.

Location

Cell surface, Secreted, Cytoplasmic

Image Legend

Western blot testing of human pancreas tissue lysate with Glycoprotein 2 antibody (clone GP2/3510) . Predicted molecular weight ~59 kDa but may be observed at higher molecular weights due to glycosylation.