CD11b Antibody / MAC-1

CD11b is a cell adhesion molecule that acts as a receptor for cell surface ligands such as intracellular adhesion molecules (ICAMs) or soluble ligands. Integrins are heterodimeric proteins that contain an a chain and b chain.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 minutes at RT

UniProt

P11215

Host

Mouse

Reactivity

Human

Immunogen

A recombinant partial protein sequence (within amino acids 900-1100) from the human protein was used as the immunogen for the CD11b antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

ITGAM/4741

Applications

IHC-P

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the CD11b antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This CD11b antibody is available for research use only.

Storage Conditions

Aliquot the CD11b antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the CD11b antibody should be determined by the researcher.

Location

Cell Surface

Image Legend

IHC staining of FFPE human spleen tissue with CD11b antibody (clone ITGAM/4741) . Inset: PBS used in place of primary Ab (secondary Ab negative control) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.