B7-H3 Antibody / CD276

T cell activation and immune function are regulated by the innate immune system through positive and negative costimulatory molecules. One such molecule, B7-H3 (B7-homolog 3, also designated B7RP-2) belongs to the B7 immunoglobulin superfamily. Soluble B7-H3 binds a putative receptor on activated T-cells that is distinct from CD28, CTLA-4, ICOS and PD-1. Widely expressed on nonlymphoid tissues, B7-H3 costimulates proliferation of both CD4+ and CD8+ T cells. The ability of B7-H3 to stimulate Th1 and cytotoxic-T cell responses suggest that it may have antitumor activity. B7-H3 interactions may play a role in regulating cell-mediated immune responses against cancer, implicating B7-H3 as a potential therapeutic tool.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 minutes at RT

UniProt

Q5ZPR3

Host

Mouse

Reactivity

Human

Immunogen

A recombinant partial protein sequence (within amino acids 100-300) from the human protein was used as the immunogen for the B7-H3 antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

B7H3/4313

Applications

IHC-P

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the B7-H3 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This B7-H3 antibody is available for research use only.

Storage Conditions

Aliquot the B7-H3 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the B7-H3 antibody should be determined by the researcher.

Location

Cytoplasm, Cell surface

Image Legend

IHC staining of FFPE human kidney carcinoma tissue with B7-H3 antibody (clone B7H3/4313) at 2ug/ml. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.