Recombinant Creatine kinase B Antibody / CKB / Brain Creatine Kinase

Creatine kinases (CK) are a large family of isoenzymes that regulate levels of ATP in subcellular compartments, where they provide ATP at sites of fluctuating energy demand by the transfer of phosphates between creatine and adenine nucleotides. CKs provide the energy of phosphate hydrolysis necessary to drive the normal function of many cellular systems. In cells, the cytosolic CK enzymes consist of two subunits, which can be either B (brain type) or M (muscle type) . There are three different isoenzymes: CKMM, CKBB and CKMB. This mAb recognizes the CKBB isoenzyme and does not react with the B subunit in CKMB.

Product Specifications

CAS Number

9000-83-3

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT, Western blot: 2-4 µg/mL

UniProt

P12277

Host

Mouse

Reactivity

Human, Mouse, Rat, Hamster, Guinea pig

Immunogen

Recombinant human full-length protein was used as the immunogen for the recombinant Creatine kinase B antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG1 k

Clone

RCKBB/8841

Type

Recombinant

Applications

IHC-P, WB

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the recombinant Creatine kinase B antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This recombinant Creatine kinase B antibody is available for research use only.

Storage Conditions

Aliquot the recombinant Creatine kinase B antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the recombinant Creatine kinase B antibody should be determined by the researcher.

Location

Cytoplasm

Image Legend

Western blot analysis of Human Brain, Mouse Brain, Rat Brain, Hamster Brain, Guinea pig Brain, Human Stomach, Mouse Stomach, Rat Stomach, Hamster Stomach and Guinea pig Stomach tissue lysates using recombinant Creatine kinase B antibody (clone rCKBB/8841) . Predicted molecular weight ~43 kDa.