Alpha-1-Antitrypsin Antibody / AAT / SERPINA1

It recognizes a protein of 54kDa, which is identified antitrypsin (AAT) . The immunohistochemical staining of AAT is useful in identification of benign and malignant hepatic tumors and yolk sac carcinomas. Positive staining for AAT is also used in detection of benign and malignant lesions of histiocytic nature. This antibody is may also useful tool in the screening of patients with cryptogenic cirrhosis or other forms of liver disease with fibrosis of uncertain origin.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT, Western blot: 2-4 µg/mL

UniProt

P01009

Host

Mouse

Reactivity

Human

Immunogen

A recombinant human Alpha-1-Antitrypsin (AAT) protein fragment was used as the immunogen for the Alpha-1-Antitrypsin antibody.

Clonality

Monoclonal

Isotype

IgG

Clone

AAT/4609

Applications

IHC-P, WB

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the Alpha-1-Antitrypsin antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This Alpha-1-Antitrypsin antibody is available for research use only.

Storage Conditions

Aliquot the Alpha-1-Antitrypsin antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the Alpha-1-Antitrypsin antibody should be determined by the researcher.

Location

Cytoplasm

Image Legend

Analysis of a HuProt (TM) microarray containing more than 19,000 full-length human proteins using Alpha-1-Antitrypsin antibody (clone AAT/4609) . Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a mAb to its intended target. A mAb is considered to specific to its intended target, if the mAb has an S-score of at least 2.5. For example, if a mAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that mAb to protein X is equal to 29.