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Alpha-1-Antitrypsin Antibody / AAT / SERPINA1

It recognizes a protein of 54kDa, which is identified antitrypsin (AAT) . The immunohistochemical staining of AAT is useful in identification of benign and malignant hepatic tumors and yolk sac carcinomas. Positive staining for AAT is also used in detection of benign and malignant lesions of histiocytic nature. This antibody is may also useful tool in the screening of patients with cryptogenic cirrhosis or other forms of liver disease with fibrosis of uncertain origin.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 minutes at RT

UniProt

P01009

Host

Mouse

Reactivity

Human

Immunogen

A recombinant partial protein sequence (within amino acids 200-400) from the human protein was used as the immunogen for the Alpha-1-Antitrypsin antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

AAT/4490

Applications

IHC-P

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the Alpha-1-Antitrypsin antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This Alpha-1-Antitrypsin antibody is available for research use only.

Storage Conditions

Aliquot the Alpha-1-Antitrypsin antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the Alpha-1-Antitrypsin antibody should be determined by the researcher.

Location

Cytoplasm

Image Legend

IHC staining of FFPE human tonsil tissue with Alpha-1-Antitrypsin antibody (clone AAT/4490) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

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