Recombinant CKB Antibody / Creatine kinase B
Creatine kinases (CK) are a large family of isoenzymes that regulate levels of ATP in subcellular compartments, where they provide ATP at sites of fluctuating energy demand by the transfer of phosphates between creatine and adenine nucleotides. CKs provide the energy of phosphate hydrolysis necessary to drive the normal function of many cellular systems. In cells, the cytosolic CK enzymes consist of two subunits, which can be either B (brain type) or M (muscle type) . There are three different isoenzymes: CKMM, CKBB and CKMB. This mAb recognizes the CKBB isoenzyme and does not react with the B subunit in CKMB.
Product Specifications
CAS Number
9000-83-3
Specifications
ELISA (Order BSA-free format for coating), Western blot: 2-4 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 minutes at RT
UniProt
P12277
Host
Mouse
Reactivity
Human, Mouse, Rat, Hamster, Guinea pig
Immunogen
Recombinant human full-length protein was used as the immunogen for the recombinant CKB antibody.
Clonality
Recombinant Monoclonal
Isotype
IgG1 k
Clone
RCKBB/8839
Type
Recombinant
Applications
ELISA, WB, IHC-P
Purity
Protein A/G affinity
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Aliquot the recombinant CKB antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This recombinant CKB antibody is available for research use only.
Storage Conditions
Aliquot the recombinant CKB antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide
Applications Notes
Optimal dilution of the recombinant CKB antibody should be determined by the researcher.
Location
Cytoplasm
Image Legend
Western blot testing of human HEK293 and Y79 cell lysate with recombinant CKB antibody. Predicted molecular weight ~43 kDa.
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