SERPINA1 Antibody / Alpha 1 Antitrypsin / AAT

It recognizes a protein of 54kDa, which is identified antitrypsin (AAT) . The immunohistochemical staining of AAT is useful in identification of benign and malignant hepatic tumors and yolk sac carcinomas. Positive staining for AAT is also used in detection of benign and malignant lesions of histiocytic nature. This antibody is may also useful tool in the screening of patients with cryptogenic cirrhosis or other forms of liver disease with fibrosis of uncertain origin.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT, Western blot: 2-4 µg/mL

UniProt

P01009

Host

Mouse

Reactivity

Human

Immunogen

A recombinant partial protein sequence (within amino acids 200-400) from the human protein was used as the immunogen for the SERPINA1 antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

AAT/6325

Applications

IHC-P, WB

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the SERPINA1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This SERPINA1 antibody is available for research use only.

Storage Conditions

Aliquot the SERPINA1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the SERPINA1 antibody should be determined by the researcher.

Location

Cytoplasm

Image Legend

IHC staining of FFPE human tonsil tissue with SERPINA1 antibody (clone AAT/6325) . Inset: PBS used in place of primary Ab (secondary Ab negative control) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.