TGF beta 3 Antibody / TGFB3

Transforming growth factor betas (TGF-betas) were originally discovered due to their ability to promote anchorage-independent growth of rat NRK fibroblasts in the presence of TGF-beta. TGF-beta 1, TGF-beta 2 and TGF-beta 3 are each synthesized as precursor proteins that are very similar in that each is cleaved to yield a 112 amino acid polypeptide that remains associated with the latent portion of the molecules. TGF-beta 3 mediates many intercellular interactions that occur during embryonic development, cell differentiation and epithelial homeostasis. TGF-beta 3 overexpresses in extramammary Paget s disease (EPD) and downregulates in Bowen s disease, indicating that its expression is a useful indicator of tumor activity. TGF-beta 3 levels strongly correlate with IGF-1 and osteocalcin levels in serum. Significant amounts of TGF-beta 3 circulation appear to be representative of TGFbeta 3 expression in bone and may in part be derived from bone. Glucocorticoids may block TGF-beta production by modulating mRNA levels and c-Jun activity.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

UniProt

P10600

Host

Mouse

Reactivity

Human

Immunogen

A recombinant partial protein sequence (within amino acids 50-250) from the human protein was used as the immunogen for the TGF beta 3 antibody.

Clonality

Monoclonal

Isotype

IgG2 κ

Clone

TGFB3/4801

Applications

IHC-P

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the TGF beta 3 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This TGF beta 3 antibody is available for research use only.

Storage Conditions

Aliquot the TGF beta 3 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the TGF beta 3 antibody should be determined by the researcher.

Location

Secreted

Image Legend

IHC staining of FFPE human renal cell carcinoma tissue with TGF beta 3 antibody (clone TGFB3/4801) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.