SKIP Antibody / SNW1 / NCoA-62

Nuclear receptor coactivator NCOA62/SNW1 (also called Nuclear Protein SkiP, SKIIP, Ski-interacting protein) is a member of the SNW gene family, encodes a coactivator that enhances transcription from some Pol II promoters. This coactivator can bind to the ligand-binding domain of the vitamin D receptor and to retinoid receptors to enhance vitamin D-, retinoic acid-, estrogen-, and glucocorticoid-mediated gene expression. It can also interact with poly (A) -binding protein 2 to directly control the expression of muscle-specific genes at the transcriptional level. Finally, the protein may be involved in oncogenesis since it interacts with a region of SKI oncoproteins that is required for transforming activity.

Product Specifications

CAS Number

9007-83-4

Specifications

Flow cytometry: 1-2ug/million cells

UniProt

Q13573

Host

Mouse

Reactivity

Human

Immunogen

A recombinant fragment of human protein was used as the immunogen for the SKIP antibody.

Clonality

Monoclonal

Isotype

IgG2a

Clone

PCRP-SNW1-2A1

Applications

FACS

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the SKIP antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This SKIP antibody is available for research use only.

Storage Conditions

Aliquot the SKIP antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the SKIP antibody should be determined by the researcher.

Location

Nucleus, cytoplasm

Image Legend

Flow cytometry testing of PFA-fixed human HeLa cells with SKIP antibody (clone PCRP-SNW1-2A1) followed by goat anti-mouse IgG-CF488 (blue), Red = unstained cells.