Periostin Antibody / POSTN

Periostin (PN), also designated osteoblast-specific factor 2 (OSF-2), is a disulfide linked protein originally isolated as a osteoblast-specific factor. Periostin is a secreted protein that binds heparin and functions as a ligand for AlphaVBeta3 and AlphaVBeta5 integrins. In preosteoblasts, Periostin acts as a cell adhesion molecule and plays a role in osteoblast recruitment, spreading and attachment. Periostin is mainly detected in lower gastrointestinal tract, aorta, stomach, placenta, uterus and breast tissues but is up-regulated in epithelial ovarian tumors and overexpressed in breast cancer. Expression of Periostin is increased by bone morphogenetic protein (BMP2) and transforming growth factor Beta 1 (TGF Beta 1) . Periostin contains a typical signal sequence, followed by a cysteine-rich domain, a fourfold repeated domain, which shows homology with the insect protein fasciclin, and a C-terminal domain.

Product Specifications

CAS Number

9007-83-4

Specifications

ELISA (Order BSA-free format for coating), Immunohistochemistry (FFPE) : 1-2 µg/mL

UniProt

Q15063

Host

Mouse

Reactivity

Human

Immunogen

A recombinant partial protein sequence (within amino acids 193-326) from the human protein was used as the immunogen for the Periostin antibody.

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

POSTN/3502

Applications

ELISA, IHC-P

Purity

Protein A/G affinity

Format

Purified

Buffer

1 mg/ml in 1X PBS; BSA free, sodium azide free

Reconstitution

Aliquot the Periostin antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This Periostin antibody is available for research use only.

Storage Conditions

Aliquot the Periostin antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

1 mg/mL in 1X PBS; BSA free, sodium azide free

Applications Notes

Optimal dilution of the Periostin antibody should be determined by the researcher.

Location

Secreted, Cytoplasm

Image Legend

Analysis of a HuProt (TM) microarray containing more than 19,000 full-length human proteins using Periostin antibody (clone POSTN/3502) . Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a mAb to its intended target. A mAb is considered to specific to its intended target, if the mAb has an S-score of at least 2.5. For example, if a mAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that mAb to protein X is equal to 29.