Cytokeratin 5/6 Antibody

Cytokeratin 5 is a basic protein that is closely related to Cytokeratin 6. They share similar tissue distribution and are found in various proportions in many non-keratinizing stratified squamous epithelia eg. tongue mucosa, as well as in basal epithelia of trachea, basal cells of epidermis, hair follicles, sebaceous and sweat glands of skin, luminal cells of the mammary gland, basal cells of prostate, urothelium, vagina and endocervical mucosa. Cytokeratins 5 and 6 are also expressed in basal cell epitheliomas, squamous cell carcinomas of skin, tongue, epiglottis and of the rectal-anal region. Cytokeratin 5/6 mAb is a useful aid for classification of epithelioid mesotheliomas.

Product Specifications

CAS Number

9000-83-3

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL

UniProt

P13647, P02538

Host

Rabbit

Reactivity

Human

Immunogen

Human Cytokeratin 5- and Cytokeratin 6-homologous recombinant protein fragments were used as the immunogen for the Cytokeratin 5/6 antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG κ

Clone

KRT5.6/8275R

Type

Recombinant

Applications

IHC-P

Purity

Protein A/G affinity

Format

Purified

Buffer

1 mg/ml in 1X PBS; BSA free, sodium azide free

Reconstitution

Aliquot the Cytokeratin 5/6 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This Cytokeratin 5/6 antibody is available for research use only.

Storage Conditions

Aliquot the Cytokeratin 5/6 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

1 mg/mL in 1X PBS; BSA free, sodium azide free

Applications Notes

Optimal dilution of the Cytokeratin 5/6 antibody should be determined by the researcher.

Location

Cytoplasm

Image Legend

IHC staining of FFPE human prostate carcinoma tissue with Cytokeratin 5/6 antibody (clone KRT5.6/8275R) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.