Cytokeratin 1 Antibody / CK1 / KRT1

Keratins are a family of intermediate filament proteins that assemble into filaments through forming heterodimers of one type I keratin (keratins 9 to 23) and one type II keratin (keratins 1 to 8) . Keratins demonstrate tissue and differentiation specific expression profiles. Cytokeratin 1 is a hetero-tetramer of two type I as well as two type II keratins, keratin-10 (KRT10) . Keratin 1 is differentiation specific keratin that is one of the predominant keratins in suprabasal keratinocytes in stratified epithelia. Mutations in keratin 1 cause epidermolytic hyperkeratosis.

Product Specifications

CAS Number

9000-83-3

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 minutes at RT

UniProt

P04264

Host

Rabbit

Reactivity

Human

Immunogen

Recombinant full-length human protein was used as the immunogen for the Cytokeratin 1 antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG κ

Clone

KRT1/8342R

Type

Recombinant

Applications

IHC-P

Purity

Protein A/G affinity

Format

Purified

Buffer

1 mg/ml in 1X PBS; BSA free, sodium azide free

Reconstitution

Aliquot the Cytokeratin 1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This Cytokeratin 1 antibody is available for research use only.

Storage Conditions

Aliquot the Cytokeratin 1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

1 mg/mL in 1X PBS; BSA free, sodium azide free

Applications Notes

Optimal dilution of the Cytokeratin 1 antibody should be determined by the researcher.

Location

Cytoplasm

Image Legend

IHC staining of FFPE human skin tissue with Cytokeratin 1 antibody (clone KRT1/8342R) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.