Cytokeratin 7 Antibody
It recognizes an intermediate filament protein (IFP) of 55kDa, which is identified as cytokeratin 7. This mAb is highly specific to cytokeratin 7 and shows no cross-reaction with other IFPs. Cytokeratin 7 is a basic cytokeratin, which is found in most glandular and transitional epithelia but not in the stratified squamous epithelia. Keratin 7 is expressed in the epithelial cells of ovary, lung, and breast but not of colon, prostate, or gastrointestinal tract. mAb to cytokeratin 7 is highly useful in distinguishing ovarian carcinomas (keratin 7+) from colon carcinomas (keratin 7-) .
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 minutes at RT
UniProt
P08729
Host
Mouse
Reactivity
Human
Immunogen
Recombinant full-length human protein was used as the immunogen for the Cytokeratin 7 antibody.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
KRT7/3733
Applications
WB, IHC-P
Purity
Protein A/G affinity
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Aliquot the Cytokeratin 7 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This Cytokeratin 7 antibody is available for research use only.
Storage Conditions
Aliquot the Cytokeratin 7 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide
Applications Notes
Optimal dilution of the Cytokeratin 7 antibody should be determined by the researcher.
Location
Cytoplasm
Image Legend
Western blot testing of human SK-BR-3 cell lysate with Cytokeratin-7 antibody (clone KRT7/3733) .
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