SHP-1 Antibody / PTPN6 / PTP1C

PTPN6, which is also designated as SHP-1 and PTP1C, is expressed primarily in hematopoietic cells and characterized by the presence of two SH2 domains N-terminal to the PTP domain. It maps to 12p13, a region commonly involved in leukemia-associated chromosomal abnormalities. SHP-1 plays a role in modulating intracellular signaling for various molecules. In T-cell lymphomas and other malignancies, SHP-1 phosphatase expression is lost, by a thus far undetermined mechanism. Preliminary studies suggest that it may be helpful in differentiating mantle/marginal zone lymphomas (SHP-1+) from follicular lymphomas (SHP-1 negative) .

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 minutes at RT

UniProt

P29350

Host

Mouse

Reactivity

Human

Immunogen

A recombinant partial protein sequence (within amino acids 395-595) from the human protein was used as the immunogen for the SHP-1 antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

PTPN6/7544

Applications

IHC-P

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the SHP-1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This SHP-1 antibody is available for research use only.

Storage Conditions

Aliquot the SHP-1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the SHP-1 antibody should be determined by the researcher.

Location

Cytoplasm, Nucleus

Image Legend

IHC staining of FFPE human tonsil tissue with SHP-1 antibody (clone PTPN6/7544) . Inset: PBS used in place of primary Ab (secondary Ab negative control) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.