NSE Gamma Antibody / Neuron Specific Enolase / ENO2
This monoclonal antibody recognizes a protein of about 50kDa, which is identified as gamma-enolase. Three isoenzymes of enolases are identified, alpha, beta and gamma. Alpha-isoform is expressed in most tissues, whereas beta-form is expressed predominantly in muscle tissue whereas gamma-enolase is found only in nervous tissue. These isoforms exist as both homodimers and heterodimers, and they play a role in converting phosphoglyceric acid to phosphenolpyruvic acid in the glycolytic pathway. NSE-gamma is a useful marker to identify peripheral nerves and tumors of neuro-endocrine origins, such as pheochromocytomas. It it be usually employed in combination with other markers such as Synaptophysin, Chromogranin A, and Neurofilament.
Product Specifications
Specifications
Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 minutes at RT, Western blot: 2-4 µg/mL
UniProt
P09104
Host
Rabbit
Reactivity
Human
Immunogen
A recombinant fragment of human NSE gamma (within amino acids 416-433) was used as the immunogen for the NSE Gamma antibody.
Clonality
Recombinant Monoclonal
Isotype
IgG κ
Clone
ENO/8632R
Type
Recombinant
Applications
IHC-P, WB
Purity
Protein A/G affinity
Format
Purified
Buffer
1 mg/ml in 1X PBS; BSA free, sodium azide free
Reconstitution
Aliquot the NSE Gamma antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This NSE Gamma antibody is available for research use only.
Storage Conditions
Aliquot the NSE Gamma antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Product Datasheet
https://www.nsjbio.com/tds-pdf/nse-gamma-antibody-neuron-specific-enolase-eno2-eno8632r-v4232
Formulation
1 mg/mL in 1X PBS; BSA free, sodium azide free
Applications Notes
Optimal dilution of the NSE Gamma antibody should be determined by the researcher.
CAS Number
9000-83-3
Location
Cytoplasm
Image Legend
Western blot testing of human HepG2 and A549 cell lysate with NSE Gamma antibody (clone ENO/8632R) . Predicted molecular weight ~47 kDa.
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