NSE Gamma Antibody / Neuron Specific Enolase / ENO2
This monoclonal antibody recognizes a protein of about 50kDa, which is identified as gamma-enolase. Three isoenzymes of enolases are identified, alpha, beta and gamma. Alpha-isoform is expressed in most tissues, whereas beta-form is expressed predominantly in muscle tissue whereas gamma-enolase is found only in nervous tissue. These isoforms exist as both homodimers and heterodimers, and they play a role in converting phosphoglyceric acid to phosphenolpyruvic acid in the glycolytic pathway. NSE-gamma is a useful marker to identify peripheral nerves and tumors of neuro-endocrine origins, such as pheochromocytomas. It it be usually employed in combination with other markers such as Synaptophysin, Chromogranin A, and Neurofilament.
Product Specifications
CAS Number
9000-83-3
Specifications
Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 minutes at RT, Western blot: 2-4 µg/mL
UniProt
P09104
Host
Rabbit
Reactivity
Human
Immunogen
A recombinant fragment of human NSE gamma (within amino acids 416-433) was used as the immunogen for the NSE Gamma antibody.
Clonality
Recombinant Monoclonal
Isotype
IgG κ
Clone
ENO/8632R
Type
Recombinant
Applications
IHC-P, WB
Purity
Protein A/G affinity
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Aliquot the NSE Gamma antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This NSE Gamma antibody is available for research use only.
Storage Conditions
Aliquot the NSE Gamma antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide
Applications Notes
Optimal dilution of the NSE Gamma antibody should be determined by the researcher.
Location
Cytoplasm
Image Legend
Western blot testing of human HepG2 and A549 cell lysate with NSE Gamma antibody (clone ENO/8632R) . Predicted molecular weight ~47 kDa.
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