ENO2 Antibody / Neuron Specific Enolase / Gamma Enolase
This monoclonal antibody recognizes a protein of about 50kDa, which is identified as gamma-enolase. Three isoenzymes of enolases are identified, alpha, beta and gamma. Alpha-isoform is expressed in most tissues, whereas beta-form is expressed predominantly in muscle tissue whereas gamma-enolase is found only in nervous tissue. These isoforms exist as both homodimers and heterodimers, and they play a role in converting phosphoglyceric acid to phosphenolpyruvic acid in the glycolytic pathway. NSE-gamma is a useful marker to identify peripheral nerves and tumors of neuro-endocrine origins, such as pheochromocytomas. It it be usually employed in combination with other markers such as Synaptophysin, Chromogranin A, and Neurofilament.
Product Specifications
CAS Number
9000-83-3
Specifications
Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 minutes at RT
UniProt
P09104
Host
Mouse
Reactivity
Human
Immunogen
A recombinant fragment of human NSE gamma (within amino acids 416-433) was used as the immunogen for the ENO2 antibody.
Clonality
Recombinant Monoclonal
Isotype
IgG1 k
Clone
RENO/8684
Type
Recombinant
Applications
IHC-P
Purity
Protein A/G affinity
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Aliquot the ENO2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This ENO2 antibody is available for research use only.
Storage Conditions
Aliquot the ENO2 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide
Applications Notes
Optimal dilution of the ENO2 antibody should be determined by the researcher.
Location
Cytoplasm
Image Legend
SDS-PAGE analysis of purified, BSA-free ENO2 antibody (clone rENO/8684) as confirmation of integrity and purity.
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